SporView Biological Indicator for Non-Liquid Loads

SporView is a self-contained Biological Indicator (BI) for monitoring steam sterilization of Non-Liquid loads, such as instruments and glassware. You can choose between two spore populations (Log 5 or Log 6) based on your local regulatory requirements.

SporView contains bacterial spores on a filter-paper carrier packaged within a small, thermoplastic culture tube. Inside the thermoplastic culture tube is a sealed-glass ampoule containing G. stearothermophilus spores and a specially-formulated culture medium that turns a dramatic yellow if spores grow.  SporeView is easy-to-use and produces visual results within 24 hours. Use with Incubator LS-2070032

INSTRUCTIONS
(Included in package)Instructions for Use

Exposure: SCBIs may be placed inside representative materials or within the chamber directly. Package or wrap product as usual, if applicable. Locate SCBIs or product in areas most difficult to sterilize, as outlined in a sterilization validation protocol or according to standard operating procedure. Run the cycle.


After sterilization or exposure, remove SCBIs or product from sterilizer.

Activation: Squeeze the sides of the unit until the glass media ampoule contained within is crushed. Ensure that the disc is immersed in the growth medium. Activate one SCBI which has not been exposed in a sterilization process as a Positive Control.

Incubation: Place the processed, activated SCBIs and the Positive Control in a vertical position in an incubator at 55°C to 60°C for a minimum of 24 hours.

Monitoring: Examine the SCBIs and record observations. All positive SCBIs should be disposed of immediately. Do not continue to incubate a positive SCBI. Continued growth may result in metabolism of amino acids in the absence of sugars, causing the pH to rise and result in color reversion that is visibly darker than a sterile unit. These should be considered as positive for growth (turbidity will be present).

For unexpected positives, it is recommended that a Gram stain be performed. Gram positive rods are characteristic of the indicator organism.

Interpretation: Control SCBI: The Positive Control SCBI should exhibit a color change to yellow and/or demonstrate turbidity. If the Positive Control does not show signs of growth, consider the test invalid.

Test SCBI: A passing sterilization cycle is indicated by no signs of turbidity and the purple color not transitioning to yellow. A failed sterilization cycle is indicated by turbidity and/or a color change to yellow.

Chemical Indicator (CI): The chemical indicating strip (along the center of the SCBI label) should transition from blue to brown/black when exposed to a steam process. Lack of color change or a partial change in color of the CI does not necessarily indicate failure. The CI does not prove efficacy of sterilization; the biological result should be used to gauge efficacy of the sterilization cycle.